Supplementary Figure 1: Pairing of eHFS with iPSD did not induce LTP in DMS slices of adult rats.

(a) Schematic illustration of LTP-inducing protocols. Presynaptic eHFS consists of 4 trains of stimuli at an interval of 20 sec, and each train contains 100 pulses at 100 Hz. eHFS was delivered alone or paired with somatic current injection-induced (iPSD) or optogenetically induced postsynaptic depolarization (oPSD). (b) Paired eHFS+iPSD did not induce LTP in DMS slices of adult rats (102.43 ± 6.47 % of baseline, t₍₆₎ = -0.38, P = 0.72; paired t test. n = 7 slices from 5 rats). Whole-cell current-clamp recordings were conducted to measure excitatory postsynaptic potentials (EPSPs). Step currents (250 pA, 1 sec) were injected through the patch pipette during four trains of eHFS. Sulpiride was bath-applied to block LTD and favor LTP induction. Data are presented as mean ± s.e.m.