Supplementary Figure 6: Repopulated microglia do not originate from blood cells.

(a-b) Confocal images and quantificative results show there are no GFP-positive repopulated microglia in the hippocampus of WT parabionts. N (mouse number) = 6, 4 and 7, respectively. p < 0.001 (WT vs GFP), p > 0.999 (WT vs parabiotic-WT) and p < 0.001 (GFP vs parabiotic-WT), respectively. (c-d) Confocal images and quantificative results show there are no GFP-positive repopulated microglia in the median eminence of WT parabionts. N (mouse number) = 6, 4 and 7, respectively. p < 0.001 (WT vs GFP), p > 0.999 (WT vs parabiotic-WT) and p < 0.001 (GFP vs parabiotic-WT), respectively. Green: GFP; red: Iba1; blue: DAPI. The data are presented as mean ± SD. N: mouse number for each group; NS: not significant; *: p < 0.05; **: p < 0.01; ***: p < 0.001. One tailed one-way ANOVA with Tukey’s post hoc. Box plot elements: square for mean, box range for percentiles at 25 and 75, inside-box line for median and whisker for min and max. Each experiment in (g-h) has been independently repeated at least twice.