Supplementary Figure 1: Inhibition of CSF1R by PLX5622 rapidly depletes brain microglia. | Nature Neuroscience

Supplementary Figure 1: Inhibition of CSF1R by PLX5622 rapidly depletes brain microglia.

From: Repopulated microglia are solely derived from the proliferation of residual microglia after acute depletion

Supplementary Figure 1: Inhibition of CSF1R by PLX5622 rapidly depletes brain microglia.

(a) Scheme of PLX5622 administration and time points for observation. (b) Representative images show microglial numbers are reduced in the brain after PLX5622 administration. Each white dot represents a microglial cell. (c) Zoom-in images of microglia in somatosensory cortex. (d) Quantification of microglial density in somatosensory cortex of normal brains and the brains on PLX5622 treatment. N (mouse number) = 8, 4, 4, 4, 3, 4, 7 and 7, respectively. PLX5622: PLX5622 formulated diet. Green: GFP; blue: DAPI. The data are presented as mean ± SD; NS: not significant; *: p < 0.05; **: p < 0.01; ***: p < 0.001. One tailed one-way ANOVA with Tukey’s post hoc. p = 0.011, p < 0.001, p < 0.001, p < 0.001, p < 0.001, p < 0.001 and p < 0.001, respectively.

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