Supplementary Figure 2: Inhibition of CSF1R by PLX5622 rapidly depletes microglia in hippocampus.

(a) Representative confocal images show microglial numbers are reduced in the hippocampus after PLX5622 administration. (b) Quantification of microglial density in hippocampus of normal brains and the brains on PLX5622 treatment. N (mouse number) = 8, 4, 4, 4, 3, 4, 7 and 7, respectively. Green: GFP; blue: DAPI. The data are presented as mean ± SD; NS: not significant; *: p < 0.05; **: p < 0.01; ***: p < 0.001. One tailed one-way ANOVA with Tukey’s post hoc. p < 0.001, p < 0.001, p < 0.001, p < 0.001, p < 0.001, p < 0.001 and p < 0.001, respectively.