Supplementary Figure 6: Sstf/f;Trpv1cre and Sstf/f;Lbx1cre mice do not exhibit itch phenotypes.
From: Circuit dissection of the role of somatostatin in itch and pain
A, Sstf/f; Trpv1cre, and B, Sstf/f; Lbx1cre mice do not display significant differences in their scratch responses to intra-dermal injection of a variety of compounds, compared to littermate controls. Data are means ±SEM (n= 7, 8, 6, 6, 6, 6, 8, 8, 6, 6, 5, 6, 6, 6, 8, 8, 8, 9, 6, and 7); no significant (ns) difference in response were observed between genotypes assessed using two-sided unpaired Student t-tests (t13 = 0.5919, t10 = 0.3334, t10 = 1.703, t14 = 0.7476, t10 = 0.011, t9 = 1.565, t10 = 0.3265, t14 = 0.7429, t15 = 0.2209, t11 = 1.273, *p = 0.5641, 0.7457, 0.1194, 0.4671, 0.9913, 0.1520, 0.7508, 0.4698, 0.8282, and 0.2292). C, testing with von Frey filaments revealed significantly lower thresholds for reflex paw withdrawal responses for Sstf/f;Trpv1cre, Sstf/f;Lbx1cre and Sstf/f;Wnt1cre mice compared to control littermates, differences assessed using two-sided unpaired Student t-tests (t10 = 2.607, 2.507, 1.581, *p = 0.0262, 0.0311, and ns p = 0.1449). Data are means ±SEM (n= 6 animals).
