Supplementary Figure 8: Synchrony of TC and NRT neuron ictal firing in the GHB model.

(a) SWC-spike-triggered averages (lines: mean; shaded areas: ±SEM) of total, burst and tonic firing for GHB TC (n=44), and WA (n=18) and WQ (n=3) NRT neurons. Note the different peak time (color coded arrows) of TC and WA NRT neurons relative to SWC spike (red vertical line) and the strong temporal overlap between the ascending phase of the curves of the two thalamic populations. Ictal burst and tonic firing peaks had similar shape and latency (to the SWC-spike) to those of total firing (Supplementary Table 2). No clear peaks are evident for WQ NRT neurons. (b) Typical examples of ictal firing XCors of a TC-WA NRT neuron pair recorded during GBL-elicited ASs. Orange lines indicate confidence intervals (5–95%) of expected correlations estimated as in Fig. 5 (Online Methods). Thirty-two pairs of simultaneously recorded TC and NRT neuron pairs had broad peaks before time-zero indicating an increased probability of NRT neurons to fire after TC neurons. Note the presence of a significant trough when TC neuron total and tonic firing (b,c), but not burst firing (d), are used for the XCors, a result similar to that in GAERS. Top left inset in (b) shows superimposed autocorrelograms for the respective TC (blue) and NRT (green) neurons calculated between −400 and +400 ms. Top right inset in (b) shows superimposed spike distribution of the respective TC (blue) and NRT (green) neuron calculated for the time period −40 to +40 ms with respect to the SWC-spike.