Supplementary Figure 9: Action potential classification and firing dynamics of GAERS cortical putative excitatory neurons.

(a) EEG trace (top) and raster plots (bottom) showing SWDs and firing times, respectively, of simultaneously recorded single cortical and TC neurons. (b) High-pass filtered traces showing typical isolated cortical action potentials. (c) Auto-correlogram of a putative excitatory cortical neuron. (d) Action potential half-widths vs trough-to-peak times for putative inhibitory (n=5) (black, FS for fast-spiking) and excitatory (n=41) (red, RS for regular spiking) cortical neurons. Average action potential waveforms are shown in the inset. (e) Total firing rate of all cortical pyramidal neurons (n=41). Horizontal dashed line indicates the start and end of SWDs in the EEG. Lines are mean and shaded areas are ±SEM. (f) XCors of total firing of 3 simultaneously recorded cortical excitatory neurons during interictal periods (black) and ASs (red). (g) Cumulative distributions of the peak of total firing for all simultaneously recorded cortical excitatory (n=41) and TC (n=43) neurons show no difference between the two neuronal populations (p=0.4892, Kolmogorov-Smirnov test).