Supplementary Figure 1: Classification of action potentials of TC and NRT neurons.

(a) Example of high-pass filtered extracellular recording traces with TC (blue) and NRT (green) neuron action potentials recorded during periods of wakefulness (top), ASs (middle) and non-REM sleep (bottom) highlight the consistency of action potential sorting across different behavioral states and levels of synchrony. (b) Firing rate of all NRT neurons during wakefulness showing the demarcation between wake-active (WA, n=13) and wake-quiescent (WQ, n=9) NRT neurons at 10 Hz (dashed black line), as originally indicated by Halassa et al. (Cell, 2014). Black lines are mean±SEM. (c) Log of the ratio of wake firing rate to sleep firing rate of NRT neurons, with all but one WA neuron having a negative value, confirms the presence of two NRT neuron groups with different firing rate during wakefulness. (d) Merged brightfield and TRITC-filtered images showing the final position of a silicone probe marked with Vybrant-Dil dye within the VB. Orange outlines indicate a portion of the NRT. (e) Burst signature of GAERS TC (blue) and NRT (green, both WA and WQ) neurons during non-REM sleep show their characteristic decelerando and accelerando-decelerando patterns, respectively (n=139 TC neurons; n=25 NRT neurons). Points are mean and error bars are ±SEM. (f) Temporal dynamics of the rate of occurrence of bursts containing ≥ 3 action potentials (purple line: mean; shaded areas: ±SEM) and action potential doublets (black) of GAERS TC neurons (n=124) before, during and after ASs. Note the strong qualitative similarities between the two firing types. Dashed red lines indicate times of start and end of SWDs in the EEG.