Supplementary Figure 1: Spatial learning, place fields and tetrode locations.
(a) Latencies during Barnes maze learning over a four day period revealed control mice (blue) learned faster than Scn2a+/- mice (red) (P = 0.0027, F = 5.19, 2-way repeated measures ANOVA, followed by post-hoc Bonferroni test P<0.01 on day 3; CTR: N = 14 animals; SCN: N = 14 animals). (b) The spatial receptive fields are shown for all recorded place cells during linear track exploration sessions. Individual grey lines represent single place cell rate maps across the entirety of the track, dark red areas indicate place fields determined as described in the methods section. Control (left) and Scn2a+/- (right) fields are ordered according to their peak position on the track. (c) Reconstructed tetrode tip locations from three animals of each genotype (blue CTR, pink Scn2a+/-) overlaid onto schematic mouse brain slices, numbers indicate posterior distances from bregma. Tetrodes circled in red were excluded as they were either poorly targeted or were devoid of place cells. All boxplots represent the median (black line) and the 25th-75th percentiles, with whiskers extending to the most extreme data points, excluding outliers which are plotted as individual crosses. *P < 0.05.
