Supplementary Figure 4: Spike waveforms and Bayesian decoding of position.
(a) Left panel shows decoding accuracy for a full linear track exploration session, with hot colors indicating highest probabilities and high probabilities along the diagonal demonstrating decoded positions accurately reflect the real world positon of the animal. Middle panel shows multiple traversals of the linear track using the Bayesian decoder, hot colors representing highest probability, red line indicates true position and is shifted vertically for clarity. Right panel shows the mean decoding error for both control (blue) and Scn2a+/- mice (red), with individual points representing single animals (P = 0.99, two-sided Wilcoxon Rank-sum; CTR: N = 7 animals; SCN: N = 7 animals). (b) Example spike waveforms recorded from control mice in CA1 stratum pyramidale, for both neurons classified as both pyramidal cells (top left) and parvalbumin-like interneurons (top right). Middle panel (blue) shows average waveforms for all pyramidal (left) and PV-like (right) neurons from control mice. Scn2a+/- average waveforms are shown in the bottom panel (red) with pyramidal left and PV-like to the right, shaded areas represent SEM. (c) Pyramidal (blue) and PV-like neurons (red) were separable into two distinct clusters based on waveform asymmetry and firing frequency, however within cell types, genotypes (control and Scn2a+/- are represented by dark and light colors respectively) were indistinguishable. (d) Example phase precession plots for three place cells from each genotype (control and Scn2a+/- in blue and red respectively), showing spikes moving to progressively earlier phases with increasing passage into place fields. (e) Histograms showing the relative frequency of all place cells relative to LFP theta, with positive values indicate phase precession of cells (P = 0.24, Z = 1.16, two-sided Wilcoxon Rank-sum; CTR: N = 7 animals, n = 224 cells; SCN: N = 7 animals, n = 274 cells). All boxplots represent the median (black line) and the 25th-75th percentiles, with whiskers extending to the most extreme data points, excluding outliers which are plotted as individual crosses. *P < 0.05.
