Supplementary Figure 8: TH+ afferents of the pPVT originate in the LC.
From: The locus coeruleus drives disinhibition in the midline thalamus via a dopaminergic mechanism
a. Representative image showing viral-assisted retrograde labelling of pPVT-projecting neurons in the ventral tegmental area (VTA). Antibody staining reveals TH-expressing neurons in VTA. Histological assessment of TH-expressing PVT-projecting neurons in the VTA was independently repeated for two mice and similar results were obtained. b. Magnified portion of the image shown in (a) depicts a single retrogradely-labelled neuron in VTA (arrow) that does not appear to be immunopositive for TH. c. Representative images from two separate experiments of viral-assisted retrograde labelling of pPVT-projecting neurons in the ventral tegmental area in LC. All neurons located within the LC displayed TH immunoreactivity. Histological assessment of TH-expressing PVT-projecting neurons in the LC was independently repeated for two mice and similar results were obtained.
