Supplementary Figure 1: Classical PKC isozyme activity during sLTP. | Nature Neuroscience

Supplementary Figure 1: Classical PKC isozyme activity during sLTP.

From: PKCα integrates spatiotemporally distinct Ca2+ and autocrine BDNF signaling to facilitate synaptic plasticity

Supplementary Figure 1

A,B) Schematic of ITRACKneg (A) and IDOCKneg (B). C,D) Overlay of mean time courses of PKCα, PKCβ, and PKCγ activity as measured by ITRACK (C; n[neurons/spines]: PKCα=32/103 (same as Figure 2D), PKCβ =13/31, PKCγ = 10/27) or IDOCKS (D; n[neurons/spines]: PKCα = 6/28(same as in Figure 2K), PKCβ = 6/24, PKCγ= 8/29). Insets: Quantification of area under the curve (mean and SEM) of PKC isozymes to each glutamate uncaging pulse (as in Figure 2C,L). One way ANOVA with Sidak’s multiple comparison compared to PKCα. E) Average translocation in response to each glutamate uncaging pulse (uncaging triggered average) of ITRACKα (same data as in C), ITRACKα in the absence of glutamate (n[neurons/spines]= 6/15) or the control sensor ITRACKα neg (n[neurons/spines]= 6/19). Mean and SEM (shaded) are shown. F) Average translocation in response to each glutamate uncaging pulse (uncaging triggered average) of IDOCKSα (same data as in D), IDOCKSα in the absence of glutamate (n[neurons/spines]= 5/12) or the control sensor IDOCKSα neg (n[neurons/spines]= 7/20). Mean and SEM (shaded) are shown.

Back to article page