Supplementary Figure 4: Genetic manipulation of piwi. | Nature Neuroscience

Supplementary Figure 4: Genetic manipulation of piwi.

From: Pathogenic tau-induced piRNA depletion promotes neuronal death through transposable element dysregulation in neurodegenerative tauopathies

Supplementary Figure 4

a, Total levels of piwi protein in whole head homogenates from Drosophila harboring RNAi transgenes targeting piwi (one-way ANOVA with Tukey’s multiple comparison test, **P<0.01, n = 6 animals per genotype). b, Cell cycle activation assayed by PCNA staining in brains of Drosophila harboring RNAi transgenes targeting piwi (one-way ANOVA with Tukey’s multiple comparison test, ****P<0.0001, n = 20 animals per genotype). c, Total transgenic tau levels in whole head homogenates from tauR406W transgenic Drosophila with and without pan-neuronal overexpression of piwi (unpaired, two-tailed Student’s t-test, n.s. = not significant, n = 6 animals per genotype). Values are mean ± s.e.m. All flies are 10 days old. Western blots are cropped in a and c, full blots are presented in Supplementary Fig. 10. Full genotypes are listed in Supplementary Table 1

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