Supplementary Figure 2: Spinal cord lymphatic and hot spots.
From: CNS lymphatic drainage and neuroinflammation are regulated by meningeal lymphatic vasculature
a, Scheme of the extension of the meningeal lymphatic vasculature in the brain and spinal cord meninges. b, Representative images of the entire CNS dorsal meninges dissected from a Prox1CreERT2XRosatdTOMATO mice (Red) after 3 weeks on tamoxifen. Scale bar = 2 mm. c, Representative images of spinal cord lymphatic organization (Lyve-1 – red) around the nerve roots (green) of the spinal cord. Scale bar = 300 µm, 10 µm (inset). d, Representative images of Prox1CreERT2XRosatdTOMATO mice (Red) after 3 weeks on tamoxifen and Lyve-1 (grey) expression by the lymphatics of the nerve roots. Scale bar = 30 µm. e, Representative images Podoplanin (green) and VEGFR3 (pink) expression by the lymphatics (Lyve-1 – grey) of the nerve roots. Scale bar = 40 µm. Images from b-e are representative of 3 independent mice. f, Representative images of the cerebellar ring lymphatics (Lyve-1 – grey). Yellow arrowheads points to button-like structures along the cerebellar lymphatics. Scale bar = 1000 µm. Representative of 5 independent mice. g, Representative images of beads distribution around the nerve roots 2h after i.c.m. or i.c.v. injection of beads. Scale bar = 2 mm. Representative of 3 independent mice per group. h-i, Representative images of the meningeal lymphatic network on the side (h) and at the base (i) of the skull. Arrows point to valve like structures characteristic of collecting lymphatics. Scale bar = 1500 µm, 400 µm (insets). Representative of 2 independent mice. j, Representative image of beads distribution at the bottom of the skull, around lymphatic vessels (Lyve-1 – red). Scale bar = 1500 µm, 400 µm (insets). Representative of 2 independent mice.
