Supplementary Figure 4: Pharmacological modulation of complex spiking in lobule simplex.
From: Coordinated cerebellar climbing fiber activity signals learned sensorimotor predictions
(a) Single unit recordings during NBQX application to LS. Top, summary of complex spike rates in control and after application of NBQX for individual cells (black; n = 9 cells, 5 mice) and the average of all cells (red). Error bars are ± SEM across cells. Bottom, same as top for simple spike rates. Note that NBQX strongly reduces complex but not simple spike rates. (b) (top) Raw epifluorescence images from parasagittal cerebellar sections showing fluorescein labeling after surface application to lobule simplex in vivo (Methods, 3 mice). (bottom) Pixel masks from the same images above. Pixels were thresholded at 30% of maximum value to visualize and quantify fluorescein labeling. (c) Summary of mediolateral fluorescein spread across sections. (n = 3 animals). Error bars are SEM across animals.
