Supplementary Figure 3: Fmr1–/– mice do not slow down as much as WT mice during preferred stimuli (Hit responses) (corresponds to data in Fig. 1e,f).

a. Workflow for ball motion analysis. b. Analysis of ball motion was done with semi-automated, custom MATLAB scripts to detect black dots painted evenly onto the polystyrene ball. c. Discriminability index for a subset of the mice shown in Fig. 1d in which we recorded running speed. d-f. Overall running speed (as determined by ball motion analysis) in WT and Fmr1-/- mice at session #1 (d), session #4 (e) and final learned session (f) for Hit + Miss trials only (corresponding to preferred stimuli). We observed a significant difference in the degree to which WT and Fmr1-/- mice slow down at session #4, but not in the final learned session. Repeated measures ANOVA F2,16 = 5.49, p = 2 × 10-5. Note there is no significant genotype differences in running speed in session 1 or in the final learned session. g-i. Change in running speed (as above). F2,16 = 2.13, p = 0.02. Shaded area in panels d-i indicate s.e.m.