Supplementary Figure 7: Selective dendritic silencing disrupts mixed selectivity in L5 neurons and the L5 network representation of object location.

a, Touch evoked response amplitudes from an example L5 neuron under control (black) and tuft silenced (red) conditions, sorted by location of whisker touch. b, Reduction in mean Ca²⁺ signal amplitude from selectively diminishing global Ca²⁺ spikes in L5 neurons (n=200 cells; two-sided, paired Student’s t-test; gray lines are individual cells and black are mean±sem). c, Touch magnitude is not changed by light (n=1968 for control and 2223 touches for light; two-sided, paired Student’s t-test). The ends of the box are upper and lower quartiles, vertical line is median and whiskers are the minimum and maximum data values. d, Reduction in location tuning in normalized mean response amplitudes from diminishing Ca²⁺ spikes, control (black) and tuft-silenced (red) conditions. Symbols are mean±sem where n=18, 66, 105, 129, 200, 146, 108, 69, 19 for locations -4 to +4 in control (left) and 16, 64, 103, 119, 200, 146, 108, 69, 19 for locations -4 to +4 in light (right). e, Nonlinear reduction in location tuning from slopes of responses amplitude as a function of touch magnitude from diminishing Ca²⁺ spikes. Symbols are mean±sem where n=10, 35, 80, 149, 135, 112, 65 for locations -3 to +3 in control (left) and 10, 35, 80, 143, 126, 112, 65 for locations -3 to +3 in light (right). In d&e light colored lines are individual cells.