Supplementary Figure 10: Human SETD5 rescue experiment. | Nature Neuroscience

Supplementary Figure 10: Human SETD5 rescue experiment.

From: Haploinsufficiency of the intellectual disability gene SETD5 disturbs developmental gene expression and cognition

Supplementary Figure 10

a, Schematics of the localization of de novo SETD5 mutations in ID and ASD patients; each mutation is colour-coded based on the study reporting the de novo mutation; mutations used in this study are highlighted in bold. b-d, Representative example of the bulk enrichment sort and single cell sorting strategy. Shape analysis scatter plots (b). To exclude doublets, live cells are gated on forward scatter/ side scatter (FSC/SSC, left) followed by SSC (middle) and FSC (right). Scatter plots (c) of primary bulk enrichment for Setd5-P2A-GFP and CRISPR/Cas9-T2A-Cerulean3 positive cells (right) compared to WT non-transfected control (left). Scatter plots (d) showing enrichment for Setd5-P2A-GFP cells over the course of the secondary bulk enrichment sort (middle) and tertiary single cell sort (right). Auto-fluorescence was measured to ensure GFP positive cells.

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