Supplementary Figure 2: Lower survival probability and developmental anomalies in Setd5^+/– animals.

a, Doughnut chart indicating that Setd5^+/− animals are born at a non-Mendelian ratio (n=585 mice, of which 387 Setd5^+/+ and 198 Setd5^+/−). b, Kaplan-Meier survival curves showing reduced survival probability of mutant animals (n is 387 for Setd5^+/+ and 198 Setd5^+/−), which seems to be slightly more pronounced in the females (n is 98 male and 100 female Setd5^+/−). c, Increased normalised brain/body weight ratio is observed in P1 male (n=9 controls, n=8 mutants) and female mutant pups (n=6 controls, n=8 mutants) as well as in adult male mutants (>P30, n=10-17) (values normalised to Setd5^+/+); *P<0.05, **P<0.01, ***P<0.001; data presented as means and SEM, as well as scatter plots (Supplementary Table 1 contains detailed statistics). d-e, Typical X-ray images of Setd5^+/− and Setd5^+/+ adult animals; craniofacial maxillary/pre-maxillary abnormalities (d, arrowheads), vertebral fusion abnormalities (d, open arrow) and teeth abnormalities (e, arrowheads) are observed in Setd5^+/− mice. The percentage of animals with each of the described phenotype is indicated as doughnut charts (n=13 mice of either genotype; data and pictures (as well as top eye picture in Fig. 1c) taken with permission from International Mouse Phenotyping Consortium, available at http://www.mousephenotype.org/data/genes/MGI:1920145).