Supplementary Figure 4: The mechanism underlying NOTCH1 upregulation of the transcription of SOX2. | Nature Neuroscience

Supplementary Figure 4: The mechanism underlying NOTCH1 upregulation of the transcription of SOX2.

From: Invasion of white matter tracts by glioma stem cells is regulated by a NOTCH1–SOX2 positive-feedback loop

Supplementary Figure 4

(a) Luciferase reporter assay of the 5’-promoter of SOX2. NOTCH1OE, GBM2 non-GSC cells stably overexpressing NOTCH1 via. pcDNA 3.1-NOTCH1; Vector, GBM2 non-GSC cells stably transfected with pcDNA3.1 empty vector; n=3 biologically independent. samples, the data are the mean±SD. (b) The similarity of the Sox2 promoter region between mouse and human. (c) The putative. binding sites of RBP-Jκ located at the promoter of the human SOX2 gene. (d) Chromatin. immunoprecipitation (ChIP) analysis in GSCs. derived from GBM2 cells. PCR primers were designed to surround predicted RBP-Jκ-binding sites 1 and 2 on the SOX2 promoter. Nonspecific IgG and anti-RNA polymerase II were used as controls. Three times these experiments were repeated independently with similar results. m, DNA ladder

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