Supplementary Figure 7: Luciferase reporter assays of the enhancers of NOTCH1. | Nature Neuroscience

Supplementary Figure 7: Luciferase reporter assays of the enhancers of NOTCH1.

From: Invasion of white matter tracts by glioma stem cells is regulated by a NOTCH1–SOX2 positive-feedback loop

Supplementary Figure 7

(A) The putative enhancer binding sites of Sox2 located in the introns and exons of the NOTCH1 gene. (B) Luciferase activity of GBM2. non-GSC cells after different fragments containing the putative enhancers were cloned into a minimal promoter of the pGL-4 luciferase. vector and then co-transfected with pcDNA 3.1-SOX2, n=3 biologically independent samples. Center line represents the median, lower. and upper limits of the box represent the 25th and 75th percentiles, and whiskers show maximum and minimum

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