Supplementary Figure 16: Immunoblots representing the distribution of TDP-43 and pTDP-43, and verification of the size of TDP-43 aggregates segregated by density-floatation gradients. | Nature Neuroscience

Supplementary Figure 16: Immunoblots representing the distribution of TDP-43 and pTDP-43, and verification of the size of TDP-43 aggregates segregated by density-floatation gradients.

From: TDP-43 extracted from frontotemporal lobar degeneration subject brains displays distinct aggregate assemblies and neurotoxic effects reflecting disease progression rates

Supplementary Figure 16: Immunoblots representing the distribution of TDP-43 and pTDP-43, and verification of the size of TDP-43 aggregates segregated by density-floatation gradients.

a-b. Representative immunoblots of the distribution of TDP-43 (a) and pTDP-43 (b) on velocity gradients. SarkoSpin-solubilized brain homogenates from frontal and motor cortex of control (black), FTLD-TDP-A (green), and FTLD-TDP-C (blue) were fractionated by density and the collected fractions (numbered from top to bottom of the gradient) were analyzed for pTDP-43 and TDP-43 content by western blotting. The experiments shown (two independent biological samples and fractionations) were repeated in total four times independently with similar results. c. Representative Native PAGE immunoblots of TDP-43 (left panels), and pTDP-43 (right panels) on density fractionations from control (black), FTLD-TDP-A (green), and FTLD-TDP-C (blue) patient brain samples. Only fractions 1 to 14 were loaded due to available wells on native PAGE gels. Density values calculated by refractometry are indicated on the top of the graph. pTDP-43-positive pathological aggregates are seen at >10 MDa for FTLD-TDP-A and FTLD-TDP-C (green and blue arrowheads respectively), whereas oligomeric TDP-43 assemblies are observed in all samples between 66 kDa and 10 MDa (black brackets). These experiments were repeated twice independently with similar results.

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