Supplementary Figure 3: Immunoblots of SarkoSpin fractions from all groups of patients show consistent isolation of pTDP-43. | Nature Neuroscience

Supplementary Figure 3: Immunoblots of SarkoSpin fractions from all groups of patients show consistent isolation of pTDP-43.

From: TDP-43 extracted from frontotemporal lobar degeneration subject brains displays distinct aggregate assemblies and neurotoxic effects reflecting disease progression rates

Supplementary Figure 3: Immunoblots of SarkoSpin fractions from all groups of patients show consistent isolation of pTDP-43.

Brain from control (black, C), ALS (red), FTLD-TDP type A (green, F-A), type B (yellow, F-B) and type C (blue, F-C) motor cortex (a, b) and frontal cortex (c) were subjected to SarkoSpin. The supernatant and pellet fractions were analyzed by SDS-PAGE and immunoblotted against TDP-43, pTDP-43, hnRNPA1 and SOD1 antibodies. Posttranslational modifications related to pathological TDP-43 are marked with asterisks: poly-ubiquitination smear (***), hyper-phosphorylation (**) and C-terminal fragmentation (*). Ssupernatant, P – pellet, L – ladder.

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