Supplementary Figure 7: BAG3 protein is differentially regulated in neurons in human non-AD and AD cases. | Nature Neuroscience

Supplementary Figure 7: BAG3 protein is differentially regulated in neurons in human non-AD and AD cases.

From: A tau homeostasis signature is linked with the cellular and regional vulnerability of excitatory neurons to tau pathology

Supplementary Figure 7

(a) Representative immunofluorescent images of the co-staining of IN neuronal marker (GAD1, purple), pan neuronal marker (NeuN, green), and BAG3 (red) in the BA9 of human AD (Braak stage V/VI) brain. The white arrow represents the IN neurons (GAD1+/NeuN+), while the white arrowhead indicates the putative EX neurons (GAD1-/NeuN+). The letter “g” stands for glial cells. The nuclei (blue) were counterstained with Hoechest3342. Three independent experiments were repeated with similar results. Scale bar, 20 µm. (b) Comparison of the mean intensity of BAG3 in individual neurons in the BA9 regions (n = 3 human brains, 20 GAD1-/NeuN+ and 20 GAD1+/NeuN+ neurons from each case). Data are presented as mean ± SEM. The statistical significance was assessed by the two-tailed nonparametric Mann-Whitney test. *** P < 0.0001 vs non-AD and/or AD EX neurons (The Mann-Whitney U is 1551, 714, 531, and 130, respectively).

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