Supplementary Figure 4: Pupil dilation following LC-NA neuron activation depends on the baseline level of arousal and optogenetic activation frequency and duration.
From: Active control of arousal by a locus coeruleus GABAergic circuit
a, Scatter plot showing the influence of baseline pupil size on pupil dilation associated with LC-NA-ChR2 neuron activation for one mouse. Light activation was on for 2 s at a frequency of 10 Hz. Trials with no laser stimulation are shown as control. b, Influence of baseline pupil size on pupil dilation following LC-NA-ChR2 activation. Data displayed as mean ± s.e.m (***P <0.001 and *P <0.05 using unpaired two-tailed t-test: t8 = 6.42, t10 = 8.86, t10 = 7.14, t10 = 2.43; N = 6 mice) c, Average pupil trace following LC-NA-ChR2 optical activation at different durations. d, Influence of the frequency or duration of LC-NA-ChR2 laser activation on pupil dilation. Box plots indicate the median (center line), first quartiles (box edges), minimum/maximum values (whiskers), and outliers (+). *P <0.05, **P <0.01, and ***P <0.001 using two-tailed paired t-test comparing the mean with zero; P = 0.291 (t5 = 1.18), P = 0.011 (t5 = 3.93), P = 0.00003 (t5 = 14.55), and P = 0.00052 (t5 = 7.91) for 3, 5, 10, and 30-Hz pulse frequencies; P = 0.0342 (t5 = 2.89), P = 0.0105 (t5 = 3.98), P = 0.00004 (t5 = 13.24), and P = 0.000004 (t5 = 21.81) for 0.1, 0.5, 1, and 2-s pulse durations. N = 6 mice.
