Supplementary Figure 9: Gain control effect of LC-GABA neurons on pupil response to tone pips is independent of tone frequency.
From: Active control of arousal by a locus coeruleus GABAergic circuit
a, Example of pupil size raster for trials with and without LC-GABA activation in a Gad2-Cre ChR2-injected mouse. Left to right columns are for different tone intensities. The bottom traces show the session average for no laser (black) and laser (green) conditions. The vertical red line indicates the tone onset. In each condition, 71–128 trials were used from 1 mouse. Data are displayed as mean ± s.e.m. b, Pupil dilation increase with tone intensity for all three frequencies tested. Gray lines show the average response per mouse; the bold color lines show a linear fit and the P value is for significance of the correlation using one-way ANOVA (F4,35 = 4.08, 3.32, and 7.19 for 8, 9, and 10-kHz frequencies). N = 8 mice. c, Average pupil response at maximum tone intensity compared for all frequencies tested. No significant difference was reported using one-way ANOVA (P = 0.1854, F2,21 = 1.83). N = 8 mice. Box plots indicate the median (center line), first quartiles (box edges), minimum/maximum values (whiskers), and outliers (+). d, Average normalized suppression of pupil dilation to tone pips produced by activation of LC-GABA in Gad2-Cre ChR2-injected mice for each frequency tested. N = 4 mice. The P value is for significance of the correlation using one-way ANOVA (F4,15 = 4.80, 2.52, and 5.79 for 8, 9, and 10-kHz frequencies). Data are displayed as mean ± s.e.m.
