Supplementary Figure 8: Multi-exposure laser speckle imaging revealed CBF increased in APP/PS1 mice within minutes of α-Ly6G administration. | Nature Neuroscience

Supplementary Figure 8: Multi-exposure laser speckle imaging revealed CBF increased in APP/PS1 mice within minutes of α-Ly6G administration.

From: Neutrophil adhesion in brain capillaries reduces cortical blood flow and impairs memory function in Alzheimer’s disease mouse models

Supplementary Figure 8: Multi-exposure laser speckle imaging revealed CBF increased in APP/PS1 mice within minutes of α-Ly6G administration.The alternative text for this image may have been generated using AI.

(a) Green light reflectance image of parietal cortex of APP/PS1 mouse. Image to the right is a expanded view of the region outlined with a yellow box. (b) Raw laser speckle image of the same region as (a) with a 10 ms exposure time. (c) Correlation time image of the same region as (a). (d) Speckle contrast values as a function of image exposure time, showing fits for regions of interest located in a surface arteriole, surface venule, or parenchymal region. The corresponding symbols in the expanded view of (c) show the locations for each fit. (e) Images and (f) plot of cerebral blood flow as a function of time after antibody injection, expressed as a fraction of the value at 5 minutes before injection for APP/PS1 mice treated with isotype control antibodies or α-Ly6G. (APP/PS1 α-Ly6G: 5 mice (2 female, 3 male); APP/PS1 Iso-Ctrl: 5 mice (1 female, 4 male); age range 9–19 months; shaded regions represent the standard deviation).

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