Supplementary Figure 9: Heterogeneous responses of all three cell classes to different aspects of movement. | Nature Neuroscience

Supplementary Figure 9: Heterogeneous responses of all three cell classes to different aspects of movement.

From: Unique contributions of parvalbumin and cholinergic interneurons in organizing striatal networks during movement

Supplementary Figure 9: Heterogeneous responses of all three cell classes to different aspects of movement.

(a) Summed population calcium activity, and the corresponding movement speed and rotation from a representative recording session from a PV-Cre mouse. Movement onsets and rotation onsets, highlighted with arrows, often co-occurred reflecting a general increase in motor output on the spherical treadmill. (b) 600 identified neurons from the animal depicted in (a) revealing the distribution of cells differentially modulated by speed, rotation, or both, and are color coded and plotted anatomically. Similar to recent reports, quantitative analysis from the full population (n = 7709 neurons) revealed that MSNs close together (<100µm) were likely to be related to the same aspects of movement (Effect of distance by neuron category; two-sided Wilcoxon rank-sum, speed sensitive, w=4.66e+06, n speed=1139 MSNs, n other=6570 MSNs, P = 1.25e-04; rotation: w=5.31e+06, n rotation=1222MSNs, n other=6487 MSNs, P = 4.30e-17; conjunctive: w=1.95e+07, n conjunctive=4706 MSNs, n other=3003 MSNs, P = 1.83e-48). (c) Population pie charts showing proportion of neurons modulated by speed alone (red), rotation alone (purple) or both (black), sorted by cell type. All three cell types are more likely to be both speed and rotation sensitive (conjunctive) than either alone. The number of conjunctive neurons differed significantly from speed alone (binomial test, all two-sided): MSN’s, P = 0, n conjunctive=4736 neurons, n speed=1144 neurons; CHI’s, P = 3.08e-07, n conjunctive=37 neurons, n speed=4 neurons; PV’s, P = 2.27e-08, n conjunctive=58 neurons, n speed=11 neurons; or rotation alone: MSN’s, P = 0, n conjunctive=4736 neurons, n rotation=1230 neurons; CHI’s, P = 8.52e-07, n conjunctive=37 neurons, n rotation=2 neurons; PV’s, P = 2.71e-11, n conjunctive=58 neurons, n rotation=6 neurons; Bonferroni-corrected p-values. Total number of MSNs: 7755 (1144 speed-related, 1230 rotation-related, 4736 conjunctive); total number of PVs: 79 (11 speed-related, 6 rotation-related, and 58 mixed); total number of CHIs: 51 (4 speed-related, 2 rotation-related, and 37 mix-related) from 6 PV-Cre mice and 6 Chat-Cre mice. *=P<0.05, **=P<0.01, ***=P<0.001.

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