Supplementary Figure 9: OPC clusters displace astrocyte end feet from vessels in PDGFRα-creERT2:APCfl/fl remyelination. | Nature Neuroscience

Supplementary Figure 9: OPC clusters displace astrocyte end feet from vessels in PDGFRα-creERT2:APCfl/fl remyelination.

From: Aberrant oligodendroglial–vascular interactions disrupt the blood–brain barrier, triggering CNS inflammation

Supplementary Figure 9

(a) Transverse section of vessels at 10dpl lesion edges following lysolecithin demyelination in spinal cord dorsal funiculus of PDGFRα-creERT2:APCfl/fl mice. Vessels are shown in blue (CD31+), astrocytes and processes in green (GFAP+) and processes of OPC perivascular clusters in red (PDGFRα+). (b-d) 3D reconstruction of image in (a) showing vessels in blue (CD31+), astrocytes and processes in green (GFAP+) and processes of OPC perivascular clusters in red (PDGFRα+). OPC perivascular clusters displace astrocyte endfeet during remyelination, and the processes of perivascular clustered OPCs appear to exist between endothelial cells and overlying astrocyte endfeet (arrows b-d). Scale bar, 10 μm. For all staining results, experiments were repeated at least three times independently with similar results.

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