Supplementary Figure 2: Immunoblot analysis of AHR, NFkBp65 and TRAF6.
From: Control of tumor-associated macrophages and T cells in glioblastoma via AHR and CD39
(a) Immunoblots of AHR (top) and GAPDH (bottom) in total protein lysates of RAW264.7 macrophages overexpressing or not the miR-29b, corresponding to the immunoblot in the Fig. 2n. Control samples (1, 3, 5), miR-29b transfected cell samples (2, 4, 6). (b) Representative immunoblots of AHR (upper panel) and GAPDH (lower panel) in total protein lysates of BMDMs from WT and AHRLysM mice, corresponding to the immunoblot in the Supplementary Fig. 3b. BMDMs WT (1, 3 and 5) and AHRLysM (2, 4 and 6) samples. (c) Western Blot analysis of NF-κB (p65) (upper panel), GAPDH (middle panel) and histone 3 (lower panel) in cytoplasmatic and nuclear fraction of BMDMs stimulated or not with TCM for 90 minutes from WT and AHRLysM mice, corresponding to the immunoblot in the Fig. 4l. Samples: 1 (control WT), 2 (TCM WT), 3 (control AHRLysM) and 4 (TCM AHRLysM). (d) Immunoblots of TRAF6 (upper panel) and GAPDH (lower panel) in total protein lysates of BMDMs stimulated or not with TCM for 6 hours from WT and AHRLysM mice, corresponding to the immunoblot in the Supplementary Fig. 4k. Samples: 1 (control WT), 2 (TCM WT), 3 (control AHRLysM) and 4 (TCM AHRLysM). L: ladder.
