Supplementary Figure 8: Stronger suppression of reward response on operant trials with an easier task.
a. Top: Trial-averaged population response of a representative field of view to random, operant, and tone-cued rewards on a different task version in which all wheel movements towards the midline were rewarded. ROIs are by mediolateral position within the field of view. Middle: Trial-averaged steering wheel velocity. Bottom: Trial-averaged licking response. Velocity and licking are shown as mean ± s.e.m. across trials (n = 11 random rewards, 210 trial rewards, and 20 tone-cued rewards). b. Scatter plots showing pairwise comparisons of response amplitude (computed as mean over 0 to +100 ms after each reward-related event) across different reward conditions. N = 556 neurons from 2 FOVs in 2 mice. Data points from representative field of view (panel a) are shown in darker gray. c. Relative response magnitude in neurons responsive to random reward (mean response over 0 to 100 ms after random reward > 2 s.d. above baseline) in mice trained on the task used throughout the majority of this study (black) and the easier task version (gray). Data are shown as mean ± s.e.m., and n = 400 neurons (of 891) for mice trained on the hard task and n = 417 neurons (of 556) for mice trained on the easy task (Kruskal-Wallis test, H = 2543, d.f. = 7, p < 1 × 10−99, significance values for Bonferroni-corrected individual comparisons: hard task vs easy task (trial reward), p = 5 × 10−25; hard task vs easy task (cued reward), p = 9 × 10−15). Statistics summary: ***p < 0.001.
