Supplementary Figure 1: TC input elimination is observed following whisker trimming and with genetic labeling of TC inputs.

a, Daily whisker trimming from P4 results in decreased barrel fluorescence intensity by day 16 (bottom panels), but no by day 6 (top panels). Scale bar, 150 µm. b, Quantification for barrel fluorescence intensity in a (Two-way ANOVA with Sidak’s post hoc, 6-day control vs trimmed, n = 5 animals, P = 0.5093, t = 1.078, df = 14; 16-day control vs trimmed, n = 4 animals, **P = 0.0071, t =3.496, df = 14). Data are normalized to the control barrel cortex within the same animal for each timepoint. c, TC inputs labeled by transgenic expression of tdTomato are eliminated in the deprived barrel cortex in a CX3CR1-dependent manner following whisker lesioning by cauterization. Representative tangential sections of Sert-Cre tdTomato labeled TC inputs within layer IV of the control and deprived barrel cortices of Cx3cr1^+/- (top row) and Cx3cr1^-/- (bottom row) mice 7 d post-sensory deprivation. Scale bar, 150 µm. d, There is a significant decrease in TC inputs as measured by fluorescence intensity of tdTomato signal in the deprived (gray bars) vs. the contralateral control (black bars) barrel cortex in Cx3cr1^+/- mice 7 d-post deprivation. No significant decrease in fluorescence was observed in Cx3cr1^-/- mice. Data are normalized to the control barrel cortex for each genotype. (Two-Way ANOVA with Sidak’s post hoc, n=4 animals per genotype, Cx3cr1^+/- control vs deprived, *P = 0.0405, t = 2.668, df = 12; Cx3cr1^-/- control vs deprived, P = 0.9808, t = 0.1782, df = 12.) All data presented as mean ± SEM.