Supplementary Figure 4: Ifngr1-/- mice display similar clinical course and viral burden as WT mice. | Nature Neuroscience

Supplementary Figure 4: Ifngr1-/- mice display similar clinical course and viral burden as WT mice.

From: T cells promote microglia-mediated synaptic elimination and cognitive dysfunction during recovery from neuropathogenic flaviviruses

Supplementary Figure 4: Ifngr1-/- mice display similar clinical course and viral burden as WT mice.

(a-f) Loss of IFNγ signaling does not affect survival, weight loss, or clinical score. (a,d) Survival analysis (n indicated in the figure). (b,e) No difference in weight loss following infection, expressed as % of day 0 body weight (b: n=4 (WT Mock), 10 (WT WNV), 5 (Ifngr1-/- WNV) mice per group; e: n=20 (WT mock), 15 (WT ZIKV), 16 (Ifngr1-/- ZIKV) mice per group, mean ± SD, b: p=0.0001, 0.0002 e: p=0.0002, 0.0001). (c,f) No difference in clinical score following infection (n as in b,e, mean ± SD). (g-i) Loss of IFNγ signaling does not influence replication or clearance of infectious virus, with the exception of slight delay in the cerebellum. Viral burden was quantified via standard plaque assays. Data are pooled from two independent experiments (n=10 mice per group; mean ± SD, i: p=0.0040). Data was analyzed by log rank (Mantel-Cox) test (a,d) or two-way ANOVA (b,c,e-i) and corrected for multiple comparisons. **, P<0.005.

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