Supplementary Figure 9: Extended data from Fig. 5. Activation of glial cells is reduced in APP;PS1;Sfrp1-/- mice. | Nature Neuroscience

Supplementary Figure 9: Extended data from Fig. 5. Activation of glial cells is reduced in APP;PS1;Sfrp1-/- mice.

From: Elevated levels of Secreted-Frizzled-Related-Protein 1 contribute to Alzheimer’s disease pathogenesis

Supplementary Figure 9

A-H) Frontal sections of the cortex from 9 months-old APP;PS1 and APP;PS1;Sfrp1-/- (n=3) mice immunostained with antibodies against LAMP1, Iba1 CD45 and Aβ42 or GFAP and stained with ThioS, as indicated in the panels. (I, J) Quantification of LAMP1 hotspots and Iba1, CD45 and GFAP immunoreactivity in 4.5 (n=3, for both genotypes) and 9 (n=3 or 4, for both genotypes) months-old cortex of APP;PS1 and APP;PS1;Sfrp1-/- mice. Data are means ± SEM and were analyzed with two-way ANOVA followed by Bonferroni test. *p≤0.05; **p≤0.01; ***p≤0.001; ****p≤0.0001. Number of analyzed animals is indicated in the bars. Scale bar: 200μm.

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