Supplementary Figure 11: mRNA and protein levels of APP, ADAM10, BACE1 and Axin2 are not affected by Sfrp1 inactivation in APP;PS1 mice. | Nature Neuroscience

Supplementary Figure 11: mRNA and protein levels of APP, ADAM10, BACE1 and Axin2 are not affected by Sfrp1 inactivation in APP;PS1 mice.

From: Elevated levels of Secreted-Frizzled-Related-Protein 1 contribute to Alzheimer’s disease pathogenesis

Supplementary Figure 11

A) RT-PCR analysis of App, Adam10, Axin2 and GAPDH mRNA from APP;PS1 (n=3) and APP;PS1;Sfrp1-/- (n=3) brains. Note that mRNA levels are similar in both mouse lines, as normalized with control GAPDH levels. B-D) Western blot analysis of BACE1 (B), ADAM10 (C) and Axin2 (D) protein levels in APP;PS1 and APP;PS1;Sfrp1-/- brains. Data were normalized to α-Tubulin levels used as a loading control. The data are means ± SEM analyzed with two-sided Student-t test; n.s. not statistically significant.

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