Supplementary Figure 6: NP tau and amyloid-β₄₂ are increased around X34+ plaques regardless of plaque size.

a, b, AT8⁺ NP tau data from Fig. 1i and k was re-analyzed as a function of average plaque size per image by dividing the total X34⁺ voxels per image by the number of X34 surfaces. Significant genotype effects were observed for seeded APPPS1-21 T2WT and T2KO (F_{(1, 111)} P<0.0001; <75000: P<0.0001, 75000-125000: P=0.0005, >125000: P<0.0001) (a) and T2CV and T2R47H (F_{(1, 84)} P<0.0001; <75000: P=0.4399, 75000-125000: P=0.0272, >125000: P<0.0001) (b) mice regardless of plaque size. c,d, amyloid-β₄₂ data from Fig. 2k and m was re-analyzed as a function of average plaque size as previously described; significant genotype effects were seen for APPPS1-21 T2WT and T2KO (F_{(2, 111)} P<0.0001; <75000 P<0.0001, 75000-125000 P=0.0004, >125000 P=0.0147) (c) and T2CV and T2R47H (F_{(1, 84)} P<0.0001; <75000 P=0.7983, 75000-125000 P=0.1178, >125000 P<0.0001) (d) mice regardless of plaque size. The number of images within each plaque size range varied; <75000 (T2WT n = 27 images, T2KO n = 22 images) (T2CV n = 9 images, T2R47H n = 13 images), 75000-125000 (T2WT n = 17 images, T2KO n = 23 images) (T2CV n = 12 images, T2R47H n = 13 images), >125000 (T2WT n = 11 images, T2KO n = 17 images) (T2CV n = 21 images, T2R47H n = 22 images). Significance reported for genotypes within each plaque size range as determined by a two-way ANOVA with Tukey post hoc test. Data are plotted as individual points with mean ± SEM.