Supplementary Figure 6: Comparison of OXT and non-OXT responses to TRPA1 stimulation (continued).
From: Zebrafish oxytocin neurons drive nocifensive behavior via brainstem premotor targets
(a) Volumetric imaging of an example Tg(HuC:GCaMP6s; oxt:Gal4; UAS:nsfb-mCherry) fish. Raster plots show Δf/f activity traces for all visible OXT and non-OXT neurons (n = 30 OXT neurons, n = 53 non-OXT neurons, 3 non-overlapping z-planes) for the entire experiment (10 UV stimuli of 100 ms duration alternating between 2 intensities (1 mW and 7 mW), 120 s inter-stimulus interval (ISI)). In this plot, neurons are sorted along the A-P axis. The same neurons were visually identified in both the DMSO and Optovin conditions. Bottom: Average Δf/f for all neurons and simultaneously recorded tail angle traces. Red dots represent UV stimuli, with larger dots representing the higher intensity stimulus. Non-OXT neurons had similar responses to UV in DMSO, but weaker responses to UV in Optovin, than OXT neurons. (b) Regressors for 6 neuron classes (corresponding to the stimulus, stimulus-locked behavior (motorstim) and spontaneous (motorspon) behavior in both DMSO and Optovin), and example OXT and non-OXT neurons from Tg(HuC:GCaMP6s) experiments that show strong correlation (Pearson’s) with each regressor. The neuron’s response in DMSO and Optovin (shaded pink) are shown. Note that each neuron may correlate with more than one regressor.
