Supplementary Figure 10: Effect of Mauthner Cell ablation on TRPA1 and ChR2-induced behaviors.

(a) The mean differences (post-pre values) in peak angle (°), maximum and mean velocity (°/s), bout duration (ms) and latency (ms) for sham and M-cell ablated fish reveal no specific deficits except in the latency of ChR2-induced behavior. All bouts were aggregated per fish. Bar plots show mean ± SEM. Fish with a post-ablation response probability of <0.5 were excluded from the kinematic analysis. *p < 0.05. Δ Peak angle: p = 0.13 (Optovin Sham), 0.31 (Optovin Ablated), 0.084 (ChR2 Sham), 0.22 (ChR2 Ablated); Δ Max velocity: p = 1 (Optovin Sham), 0.69 (Optovin Ablated), 0.28 (ChR2 Sham), 0.56 (ChR2 Ablated); Δ Mean velocity: p = 0.20 (Optovin Sham), 0.84 (Optovin Ablated), 0.49 (ChR2 Sham), 0.31 (ChR2 Ablated); Δ Duration: p = *0.012 (Optovin Sham), 0.69 (Optovin Ablated), 1 (ChR2 Sham), 0.84 (ChR2 Ablated); Δ Latency: p = 0.91 (Optovin Sham), 0.0625 (Optovin Ablated), 0.85 (ChR2 Sham), *0.03 (ChR2 Ablated); N = 9 (Optovin Sham), 6 (Optovin Ablated), 10 (ChR2 Sham), 6 (ChR2 Ablated) fish, two-sided Wilcoxon rank-sum test. (b) Bootstrapped mean differences confirm an increase in ChR2 response latencies (mean = +359 ms, 95%CI [+134 613], ***p= 4x10^-4) in ablated (red) but not sham (blue) fish. TRPA1 (Optovin) response latencies also appeared to be significantly decreased (*p=0.02) using this permutation test. Black line indicates zero mean difference. All bouts extracted per fish, as well as the set of fish, were resampled with replacement for 10000 iterations to generate a bootstrapped distribution of mean differences (post-pre values) between pre and post ablation behavior. P<0.05 (one-tailed) if % below (or above) zero is less than 5%. * = p < 0.05, **p<0.01, ***p<0.001.