Supplementary Figure 11: Nitroreductase-mediated ablation of OXT neurons.
From: Zebrafish oxytocin neurons drive nocifensive behavior via brainstem premotor targets
(a) Effective ablation of Tg(oxt:Gal4;UAS:nfsb-mCherry)-labeled neurons using MTZ. Representative images are shown of non-ablated (left) and ablated fish brains (right). Scale bar = 20 μm. Fish were treated with MTZ from 4-6 dpf, and their behavior was tested at 8 dpf. Note that due to variability in expression, ablation of OXT population is likely to be partial (~50-75%). This experiment was performed on more than 40 fish with similar results.
