Supplementary Figure 9: Effects of optogenetic manipulation of the mPFC–vlPAG circuit on c-fos expression in the vlPAG of SNI mice. | Nature Neuroscience

Supplementary Figure 9: Effects of optogenetic manipulation of the mPFC–vlPAG circuit on c-fos expression in the vlPAG of SNI mice.

From: A neuronal circuit for activating descending modulation of neuropathic pain

Supplementary Figure 9: Effects of optogenetic manipulation of the mPFC–vlPAG circuit on c-fos expression in the vlPAG of SNI mice.

a, b, Colocalization (see arrows) of c-fos with CaMKIIα (a, n = 4 mice) and GAD67 (b, n = 3 mice for no light and n = 4 mice for blue light) in the vlPAG without (upper panel) and with (lower panel) blue light stimulation of mPFC inputs. Scale bar: 20 μm. c, d, Quantification for the percentage of activated glutamatergic (c, p = 0.0106, t = 3.654) and GABAergic (d, p = 0.0205, t = 3.334) neurons in the vlPAG following ChR2 activation. Data are presented as mean ± SEM. A two-tailed unpaired t-test was used for (c) and (d). Numbers in parentheses denote numbers of mice for c and d.

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