Supplementary Figure 1: Analysis of nerve injury-induced changes within the endocannabinoid system in the prelimbic area of the mPFC.
From: A neuronal circuit for activating descending modulation of neuropathic pain
a-d, Quantitative PCR analysis of the expression of enzymes involved in endocannabinoid metabolism in the prelimbic area of the mPFC. mRNA levels of NAPE-PLD (a), FAAH (b), MAGL (c) and DAGL (d) in the prelimbic contralateral mPFC at 2 weeks and 4 weeks after Sham and SNI surgery (p = 0.0235, t = 2.489 for 4 weeks in a and all other comparisons are p > 0.1). Data were normalized to β-actin mRNA levels and are presented as mean ± SEM. Two-tailed unpaired t-tests were used for (a-d). Numbers in parentheses reflect numbers of mice for all panels. Asterisks denote significant differences. e, Uncropped Western blot corresponding to the data in main Fig. 1k.
