Supplementary Figure 5: Photoactivation of vCA1-engram increases GCaMP fluorescence of AcbC-engram simultaneously. | Nature Neuroscience

Supplementary Figure 5: Photoactivation of vCA1-engram increases GCaMP fluorescence of AcbC-engram simultaneously.

From: A ventral CA1 to nucleus accumbens core engram circuit mediates conditioned place preference for cocaine

Supplementary Figure 5

(a-c) AAV TRE-ChR2-mCherry was infused into the vCA1, and AAVs TRE3g-Cre, DIO-GCaMP6s, and hSyn-mCherry were infused into the AcbC of c-fos-tTA mice. Optical fibers were implanted in the vCA1 and AcbC, and GCaMP6s response to photostimulation of vCA1 were recorded in AcbC on Day 5. (a) Behavioral scheme. (b) Schematic of virus injection, photostimulation and fluorescence recordings. (c,d) Heatmaps illustrate the mCherry or GCaMP fluorescence (ΔF/F %) of 90 trials per mice from -2 s to 10 s in response to photostimuation (473 nm laser, a train of ten 15-ms light pulses at 20 Hz every 10 s for 15 min, blue vertical bars). Peri-event plots illustrate the averaged fluorescence of each mice. The curves and shaded regions indicate the mean ± s.e.m. [n = 7 mice].

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