Supplementary Figure 9: Anatomic engram connection from vCA1 to AcbC. | Nature Neuroscience

Supplementary Figure 9: Anatomic engram connection from vCA1 to AcbC.

From: A ventral CA1 to nucleus accumbens core engram circuit mediates conditioned place preference for cocaine

Supplementary Figure 9

(a-c) AAV DIO-WGA was infused into the AcbC of D1-Cre (a) or D2-Cre (b) mice. (a, b) Strategy for retrograde labeling and representative images. The broken white lines show the position of AC. (c) Summary bar graph of WGA+ cells counts in the vCA1. [D1-Cre n = 10, D2-Cre n = 12, Kruskal-Wallis One-way ANOVA on ranks, H = 15.110 with 1 degrees of freedom, p = 0.000101] ***p < 0.001. (d-f) Retrograde labelling with rabies-virus system. AAV EF1α-DIO-his-EGFP-2a-TVA and AAV-EF1α-DIO-RVG were injected in the AcbC of D1-Cre or D2-Cre mice, followed by RV-ENVA-deltaG-dsRed injection 2 weeks later. (d, e) Strategy for retrograde labeling and representative images of starter cells in the AcbC and RVdG-dsRed+ cells in the vCA1 that provide direct monosynaptic inputs on D1-MSNs (d) and D2-MSNs (e). The broken white lines show the position of AC. (f) Summary bar graph of input connection strength index (numbers of labeled presynaptic neurons / numbers of starter neurons) in the vCA1. [D1-Cre n = 5, D2-Cre n = 4, Kruskal-Wallis One-way ANOVA on ranks, H = 6.000 with 1 degrees of freedom, p = 0.014] *p < 0.05. (g, h) Anterograde labelling with scAAV system. scAAV2/1-hSyn-Cre was injected in the vCA1 of Ai14 mice and smFISH was performed with probes of drd1, drd2, and tdTomato. (g) Strategy for anterograde labeling and representative image. (h) Summary bar graph of Drd1+ tdTomato+ and Drd2+ tdTomato+ cell counts in the AcbC. [n = 5, One-way ANOVA, F (1, 8) = 158.833, p = 0.000001] ***p < 0.001. Bar graphs show mean ± s.e.m. and individual data (circles).

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