Extended Data Fig. 7: SPP1 immunohistochemistry and clusterwise SPP1 expression across age bins - control samples.
From: Mapping microglia states in the human brain through the integration of high-dimensional techniques

a) Immunohistochemistry of SPP1 and Iba1 in control grey and white matter. Each image is representative of n=5 independent images taken per specimen. b) Violin and box plots showing the transcript counts of SPP1 across age bins and. Statistically significant p-values are indicated resulting from two-way analysis of variance followed by a Tukey post-hoc test. Violin plots represent kernel density estimates of expression values from n=4,396 cells from 15 samples. The boxplot spans the 25th to 75th percentiles and the whiskers represent 1.5 times the interquartile range. The bold line indicates the median.