Supplementary Fig. 10: Effects of paRac1 stimulation on cue-induced nose poke responding in saline controls.
From: Silent synapses dictate cocaine memory destabilization and reconsolidation
(a) Diagram showing the experimental timeline and setup. (b) Summary showing that saline-trained rats with photostimulation of intra-NAcSh paRac1 and photostimulation of intra-NAcSh C450M (control) during cue re-exposure exhibited similar cue-induced seeking, measured 6 hr after cue re-exposure (C450 active = 17.88 ± 2.95, n = 8 animals; paRac1 active = 21.83 ± 4.31, n = 6 animals; C450 inactive = 5.13 ± 0.915, n = 8 animals; paRac1 inactive = 9.17 ± 2.60, n = 6 animals, F1,12=0.0002, p=0.9878, two-way RM ANOVA, withdrawal day 45 hole x virus interaction, n.s. > 0.05). (c) Nose poke responding during the 10-min cue re-exposure session in saline-trained rats whose behavioral results presented in Supplementary Fig. 10b. Rats with intra-NAcSh stimulation of paRac1 and intra-NAcSh stimulation of C450M exhibited similar nose poke responding during the cue re-exposure session (C450M = 6.00 ± 0.707, n = 8 animals; paRac1 = 5.67 ± 1.48, n = 6 animals, t12=0.22, p=0.8286, two-tail, unpaired t-test, n.s. > 0.05). (d) Nose poke responding during the 10-min cue re-exposure session in cocaine-trained rats whose behavioral results are presented in Fig. 6q. Rats with intra-NAcSh stimulation of paRac1 and intra-NAcSh stimulation of C450M exhibited similar nose poke responding during the cue re-exposure session (C450M = 18.78 ± 2.52, n = 9 animals; paRac1 = 18.38 ± 1.97, n = 8 animals, t15=0.12, p=0.9033, two-tail, unpaired t-test, n.s. > 0.05). (e) Diagram showing the experimental timeline and setup. (f) Summary showing that rats with NAcSh paRac1 and rats with NAcSh C450M (control) receiving intra-NAcSh photostimulation 2 hr after cue re-exposure exhibited similar cue-induced nose poke responding, measured 6 hr after cue re-exposure (C450 active = 23.43 ± 7.57, n = 7 animals; paRac1 active = 17.43 ± 3.72, n = 7 animals; C450 inactive = 7.57 ± 2.31, n = 7 animals; paRac1 inactive = 5.71 ± 1.96, n = 7 animals, F1,12=0.23, p=0.6370, two-way RM ANOVA, withdrawal day 45 hole x virus interaction, n.s. > 0.05). (g) Nose poke responding during the 10-min cue re-exposure session in saline-trained rats used in experiments in Supplementary Fig. 10f. Rats with intra-NAcSh paRac1 and intra-NAcSh C450M exhibited similar nose poke responding over the cue re-exposure session (C450M = 11.29 ± 1.92, n = 7 animals; paRac1 = 10.57 ± 1.96, n =7 animals, t12=0.26, p=0.7993, two-tail, unpaired t-test, n.s. > 0.05). (h) Nose poke responding during the 10-min cue re-exposure session in cocaine-trained rats whose behavioral results are presented in Fig. 6r. Rats with intra-NAcSh paRac1 and intra-NAcSh C450M exhibited similar nose poke responding during the cue re-exposure session (C450M = 28.63 ± 2.16, n = 8 animals; paRac1 = 25.80 ± 1.79, n = 10 animals, t16=1.02, p=0.3246, two-tail, unpaired t-test, n.s. > 0.05). (i) Nose poke responding during the 10-min cue re-exposure session in cocaine-trained rats whose behavioral results are presented in Fig. 6s. Rats with intra-NAcSh paRac1 and intra-NAcSh C450M exhibited similar nose poke responding during the cue re-exposure session (C450M = 27.50 ± 2.31, n = 8 animals; paRac1 = 26.00 ± 2.00, n = 8 animals, t14=0.42, p=0.6308, two-tail, unpaired t-test, n.s. > 0.05). Data presented as mean±SEM.
