Supplementary Fig. 12: Viral localization and expression for 2 hr post photostimulation in behavioral experiments.

(a-c) Example image showing HSV-mediated expression of C450M in the NAcSh and location of the optical fiber that delivered laser stimulation (a), and a magnified portion of the image showing viral expression in individual neurons (b) from a saline-trained rats used in behavioral experiments presented in Supplementary Fig. 10f. Viral expression and fiber tip localizations for all rats that were included in data analysis are presented in (c). (d-f) Example image showing HSV-mediated expression of C450M in the NAcSh and location of the optical fiber that delivered laser stimulation (d), and a magnified portion of the image showing viral expression in individual neurons (e) from a cocaine-trained rats used in behavioral experiments presented in Fig. 6r. Viral expression and fiber tip localization for all rats that were included in data analysis are presented in (f). (g-i) Example image showing HSV-mediated expression of paRac1 in the NAcSh and location of the optical fiber that delivered laser stimulation (g), and a magnified portion of image showing viral expression in individual neurons (h) from a saline-trained rat used in behavioral experiments presented in Supplementary Fig. 10f. Viral expression and fiber tip localizations for all rats that were included in data analysis are presented in (i). (j-l) Example image showing HSV-mediated expression of paRac1 in the NAcSh and location of the optical fiber that delivered laser stimulation (j), and a magnified portion of the image showing viral expression in individual neurons (k) from a cocaine-trained rat used in behavioral experiments presented in Fig. 6r. Viral expression and fiber tip localizations for all rats that were included in data analysis are presented in (l).