Supplementary Fig. 1: Training results and example traces for silent synapse experiments in Fig. 1.
From: Silent synapses dictate cocaine memory destabilization and reconsolidation
(a) Diagram showing the timeline of self-administration training with two withdrawal time points at which the behavioral and electrophysiological experiments were performed. (b-e) Self-administration training results from rats whose electrophysiology results are presented in Fig. 1f–h (saline 1w/d: infusions - d1 = 18.63 ± 3.73, d2 = 10.88 ± 2.82, d3 = 11.14 ± 2.31, d4 = 9.86 ± 5.62, d5 = 12.21 ± 3.01, inactive – d1 = 12.88 ± 2.93, d2 = 9.63 ± 3.29, d3 = 12.00 ± 3.98, d4 = 9.00 ± 5.46, d5 = 8.89 ± 2.37, n = 5 animals; cocaine 1w/d: infusions – d1 = 34.60 ± 3.71, d2 = 29.40 ± 5.84, d3 = 31.20 ± 3.85, d4 = 33.00 ± 2.88, d5 = 35.00 ± 4.00, inactive – d1 = 25.80 ± 10.52, d2 = 16.67 ± 9.27, d3 = 14.60 ± 5.73, d4 = 10.00 ± 2.59, d5 = 12.60 ± 3.44, n = 6 animals; saline 45w/d: infusion – d1=13.70 ± 2.60, d2 = 11.90 ± 2.60, d3= 8.40 ± 2.30, d4 = 8.90 ± 1.90, d5 = 9.30 ± 2.30, inactive – d1= 12.20 ± 3.0, d2 = 9.30 ± 2.00, d3 = 6.30 ± 1.60, d4 = 7.00 ± 1.20, d5 = 5.80 ± 1.10, n = 5 animals; cocaine 45w/d: infusions – d1 = 19.70 ± 3.03, d2 = 21.45 ± 3.26, d3 = 20.64 ± 3.37, d4 = 22.36 ± 4.00, d5 = 21.36 ± 1.38, inactive – d1 = 7.10 ± 3.49, d2 = 7.45 ± 2.53, d3 = 4.64 ± 1.43, d4 = 6.00 ± 1.22, d5 = 11.36 ± 2.62, n = 12 animals). (f-h) Example EPSCs over 100 trials from the minimal stimulation assay for the summarized results presented in Fig. 1h. (i) Diagram showing different time points after cue re-exposure at which silent synapses were assessed. (j-o) Self-administration training results of rats whose electrophysiology results are presented in Fig. 1j–m, s–v (saline re-exp: infusions – d1 = 13.80 ± 1.72, d2 = 12.40 ± 3.44, d3 = 7.20 ± 1.32, d4 = 8.60 ± 2.14, d5 = 10.40 ± 3.66, inactive – d1 = 10.20 ± 0.735, d2 = 11.80 ± 3.07, d3 = 7.60 ± 0.678, d4 = 6.60 ± 1.29, d5 = 6.60 ± 2.21, n = 5 animals; cocaine 45w/d: infusions – d1 = 27.73 ± 3.34, d2 = 23.60 ± 2.53, d3 = 24.73 ± 2.54, d4 = 22.00 ± 1.77, d5 = 23.53 = 2.03, inactive – d1= 5.71 ± 1.48, d2 = 8.13 ± 3.07, d3 = 7.14 ± 2.38, d4 = 5.86 ± 2.29, d5 = 4.33 ± 1.41, n = 16 animals; cocaine re-exp: infusions – d1 = 32.91 ± 5.17, d2 = 30.05 ± 3.78, d3 = 26.09 ± 2.06, d4 = 26.46 ± 2.33, d5 = 32.67 ± 4.34, inactive – d1 = 12.95 ± 4.10, d2 = 16.18 ± 5.16, d3 = 8.14 ± 2.79, d4 = 8.14 ± 2.80, d5 = 9.38 ± 3.15, n = 22 animals; cocaine 2hr: infusions – d1 = 40.20 ± 3.56, d2 = 36.40 ± 2.62, d3 = 30.20 ± 2.80, d4 = 34.80 ± 2.44, d5 = 36.20 ± 4.21, inactive – d1 = 7.00 ± 3.56, d2 = 8.00 ± 3.27, d3 = 8.00 ± 2.85, d4 = 8.00 ± 3.35, d5 = 6.60 ± 2.60, n = 5 animals; cocaine 4hr: infusions – d1 = 28.86 ± 1.49, d2 = 30.14 ± 4.26, d3 = 22.43 ± 1.07, d4 = 22.57 ± 2.26, d5 = 23.57 ± 2.99, inactive – d1= 13.71 ± 4.85, d2 = 11.86 ± 4.04, d3 = 6.57 ± 1.82, d4 = 9.34 ± 2.51, d5 = 8.14 ± 2.12, n = 7 animals; cocaine 6hr: infusions – d1 = 27.15 ± 3.52, d2 = 26.92 ± 2.18, d3 = 31.23 ± 6.20, d4 = 30.46 ± 4.26, d5 = 25.69 ± 2.36, inactive – d1 = 17.62 ± 11.29, d2 = 7.54 ± 4.49, d3 = 2.75 ± 1.18, d4 = 3.39 ± 2.03, d5 = 1.60 ± 0.743, n = 13 animals). (p) Summary showing no difference in nose poke responding during the 10-min cue re-exposure session in rats in which NAcSh silent synapses were assessed 10 min, 2 hr, 4 hr, and 6 hr after cue re-exposure (Fig. 1v) (10min = 29.45 ± 3.44, n = 22 animals; 2hr = 37.40 ± 3.98, n = 5 animals; 4hr = 21.86 ± 3.12, n = 7 animals; 6hr = 31.92 ± 2.68, n = 13 animals, F3,43=1.55, p=0.22, one-way ANOVA, n.s. > 0.05). (q-s) Example EPSCs over 100 trials from the minimal stimulation assay for the summarized results presented in Fig. 1m, v. Data presented as mean±SEM.
