Extended Data Fig. 6: Characterization of mice expressing phosphomutant eIF2α in LA CamK2α principal neurons. | Nature Neuroscience

Extended Data Fig. 6: Characterization of mice expressing phosphomutant eIF2α in LA CamK2α principal neurons.

From: Cell-type-specific drug-inducible protein synthesis inhibition demonstrates that memory consolidation requires rapid neuronal translation

Extended Data Fig. 6: Characterization of mice expressing phosphomutant eIF2α in LA CamK2α principal neurons.The alternative text for this image may have been generated using AI.

a) eIF2α phosphorylation at S51 was significantly reduced in GFP+ neurons in CamK2α.eIF2α (A/A) mice compared to GFP- neurons, as well as GFP+ neurons in CamK2α.WT mice (****p<0.001). (n = 60–74 per group, 3 animals); One-way ANOVA with Bonferroni’s post-hoc test. F (2, 154) = 1055, ****p<0.0001. b) Representative motion traces from the open field test for CamK2α.WT, CamK2α.eIF2α(A/+) and CamK2α eIF2α (A/A) mice. c) In the open field test, CamK2α.eIF2α (A/A) mice acclimated to the novel environment and had comparable spontaneous locomotion compared to the CamK2α.WT mice and CamK2α. eIF2α (A/+) mice. RM One-way ANOVA. d) Bar graphs representing thigmotaxis, i.e. %time spent in center compared to total distance traversed in the open field arena for the three groups. One-way ANOVA. f) In the elevated plus maze, CamK2α.eIF2α (A/A) mice spent a significantly higher duration in the open arm compared to CamK2α WT mice (g) (*p<0.05) indicating anxiety like behavior, even though they made equivalent entries to the open arm. One-way ANOVA with Bonferroni’s post-hoc test. F(2,17) = 3.775, *p=0.0440. Data are presented as +/- SEM.

Back to article page