Extended Data Fig. 1: Validation of our CreOFF vectors. | Nature Neuroscience

Extended Data Fig. 1: Validation of our CreOFF vectors.

From: Two genetically, anatomically and functionally distinct cell types segregate across anteroposterior axis of paraventricular thalamus

Extended Data Fig. 1

Top: Representative images from an RNAscope ISH experiment used to validate our FlexOFF (a.k.a. CreOFF) construct (produced by Vector Biolabs). AAV2-EF1a-FLEX/OFF-hChR2(H134R)-eYFP was injected in the PVT of a Drd2-Cre mouse and allowed to express for two weeks. Notice that ChR2-eYFP is almost exclusively (~ 95%) expressed in Drd2-negative cells (arrows). Data from 1 mouse, with the experiment independently repeated twice. Similar results were obtained on both occasions. Bottom: Representative images from an immunohistochemistry experiment used to validate our DO (CreOFF) construct (cloned and packaged by Charu Ramakrishnan). AAV9-Syn-DO-GCaMP6s was injected in the PVT of a Drd2-Cre mouse and allowed to express for two weeks. An antibody against D2R (see Methods) was used to label D2R+ regions of the PVT. Notice that GCaMP6s expression is selectively observed in areas where D2 labeling is low or absent (arrows). Data from 1 mouse.

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