Extended Data Fig. 4: Time-lapse imaging of OPC population dynamics. | Nature Neuroscience

Extended Data Fig. 4: Time-lapse imaging of OPC population dynamics.

From: Functionally distinct subgroups of oligodendrocyte precursor cells integrate neural activity and execute myelin formation

Extended Data Fig. 4: Time-lapse imaging of OPC population dynamics.

a, Overview images of transgenic zebrafish labeling nuclei of OPCs (olig1:nls-mApple) and myelinating oligodendrocytes (mbp:nls-EGFP) at the beginning and end of a timelapse between 3 and 5 d.p.f. Dashed boxes indicate the areas shown in panel c (n = 3 animals in two experiments). Scale bar, 10 µm. b, Quantification of the fates of OPCs found in neuron-rich areas. A detailed breakdown of the data shown in Fig. 4e. c, Zoom-ins and false coloring of the time-lapse in a, showing potential behaviors of OPCs in neuron-rich areas: remaining quiescent (red cell), generating new OPCs in neuron-rich areas (magenta cells) or generating new OPCs in axo-dendritic areas (green cells). The insets at the first and last timepoints show the absence of myelin markers (mbp:nls-EGFP) in the cells studied (n = 3 animals in two experiments). Scale bar, 10 µm.

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